This manual describes the use of the transfer model to estimate the cadmium concentration in pig kidney and liver based on oral exposure to feed contaminated with cadmium. The model has several input fields (left side) and output fields (right side). The input and results can be saved using the button “Report (*.docx) ”. Source information and a citation suggestion are provided (top).
Step1: Contamination level of feed
The user can provide the contamination level (expressed in mg cadmium/kg feed) in the contamination field.
Step 2: Feed intake
The user first has to select the type of feed that is contaminated with cadmium.
a) This can either be:
- Total compound feed (blend of various raw materials and additives)
- Wet mixes (mixture of compound feed, solid and liquid by-products and water)
- Compound feed ingredients
When compound feed ingredients are selected, a drop down menu will appear to select the contaminated compound feed ingredient.
b) After selecting the contaminated feed (ingredient), default feed intake values are provided (in kg dry matter per day) for three age categories of the pig (<15 weeks, 15 – 20 weeks and > 20 weeks)1. The user can adjust these default values to the values corresponding to the actual feeding regime.
Step 3: Exposure period of pig to contaminated feed
To estimate the concentration of cadmium in the pig kidney and liver, the user has to enter the age of the pig at the beginning of the exposure period, the duration of the exposure of the animals to the contaminated feed (ingredient) and the time period after the exposure stopped (all expressed in weeks). The age at the beginning of the exposure period has to be at least 12 weeks.
Step 4: Regulatory limit (optional)
Regulatory limits (for instance maximum levels) (expressed in mg cadmium/kg tissue) can be inserted for liver or kidney. These limits will be displayed in the output graphs on the screen, but not yet in the report.
Step 5: Results
A graph with the cadmium concentration versus time will be generated for both kidney and liver (expressed in mg/kg tissue). These cadmium concentrations are valid for the average pig. The user can switch between both graphs using the button below the graph.
Step 6: Saving input and output
By using the “Report (*.docx) ” button, present in the left corner, input data and the results of the model run can be stored locally as a report file in word. The optional regulatory limits are not yet visible in the report. Input data and results are not stored at the website.
Step 7: Citation suggestion
At the top of the model page, the reference that describe the model is listed, as well as a citation suggestion. Please use the suggested citation, when referring to the model.
1 Van Raamsdonk LWD, Kan CA, Meijer GAL, Kemme PA (2007) Key figures for some farm animals and their consumption patterns [in Dutch]; https://research.wur.nl/en/publications/kengetallen-van-enkele-landbouwhuisdieren-en-hun-consumptiepatron.